The ZX-1281e has 32 I/O lines. If you need more, you may want to read
AN-203 I/O Expansion Techniques.
The mega1281 on the ZX-1281e can access up to 64K of RAM (16-bit address) using "Option ExtRamConfig On". If the option id off then only the internal 4K RAM is available. External ram is accessed using 19 I/O lines from ports A, C, and G. If you want more than 64K, you will need to employ "bank switching" and additional I/O lines to switch the bank.
The RAM daughterboard is hard-wired for 64K. The extension I/O card has up to 128K that is bank-switched via the A16 pin. It may be possible to combine two extension I/O cards to provide a total of 256K which would require 2 or 3 external lines for bank switching.